Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

ME-1

Primary Tissue

Bone Marrow

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

ME-1 cells

cell line

ME-1

cell type

Human inv(16) leukemia cell line

treated with

DMSO

chip antibody

Anti-Histone H3 (acetyl K27) antibody

chip antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and RUNX1-DNA and histone-DNA complexes were isolated with antibodies. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

48819102

Reads aligned (%)

99.2

Duplicates removed (%)

17.4

Number of peaks

41700 (qval < 1E-05)

hg19

Number of total reads

48819102

Reads aligned (%)

98.7

Duplicates removed (%)

17.7

Number of peaks

41716 (qval < 1E-05)