Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

S2 Cell line

cell line

late embryo-derived cell line

strain

S2-DRSC

genotype

wild type

treatment

10 microMolar Cu2SO4

chip antibody

Pol II Ser-2 clone 3E10 (Merck Millipore; ref 04-1571)

input dna code

B

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was prepared according to the modENCODE procedure; RNA was extracted with Qiagen Rneasy kit DNA libraries were prepared according to Illumina's instructions; RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 4000

dm6

Number of total reads

24550275

Reads aligned (%)

68.4

Duplicates removed (%)

42.0

Number of peaks

4360 (qval < 1E-05)

dm3

Number of total reads

24550275

Reads aligned (%)

69.0

Duplicates removed (%)

39.5

Number of peaks

5153 (qval < 1E-05)