SRX3000112
GSM2701960: MEF+Ascl1 36hr B2 rep1; Mus musculus; ATAC-seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
MEF+Ascl1 36hr
genotype
Tau::EGFP+/-
cell type
E13.5 fibroblast
time post dox
36 hours

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were trypsinzed in 0.05% trypsin and processed at different time points post-dox. From day 5, cells were FAC sorted by TauEGFP. ATAC-seq protocol outlined by Buenrostro, et al (Nat. Methods, 2013)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
33127802
Reads aligned (%)
91.1
Duplicates removed (%)
18.2
Number of peaks
2068 (qval < 1E-05)

mm9

Number of total reads
33127802
Reads aligned (%)
91.0
Duplicates removed (%)
18.3
Number of peaks
2028 (qval < 1E-05)

Base call quality data from DBCLS SRA