Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEF+Ascl1 24hr

genotype

Tau::EGFP+/-

cell type

E13.5 fibroblast

time post dox

24 hours

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells were trypsinzed in 0.05% trypsin and processed at different time points post-dox. From day 5, cells were FAC sorted by TauEGFP. ATAC-seq protocol outlined by Buenrostro, et al (Nat. Methods, 2013)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

51082726

Reads aligned (%)

89.1

Duplicates removed (%)

58.3

Number of peaks

13535 (qval < 1E-05)

mm9

Number of total reads

51082726

Reads aligned (%)

89.0

Duplicates removed (%)

58.4

Number of peaks

13550 (qval < 1E-05)