GSM2700101: hTert-2-H3K27ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
hTERT immortalised human skin fibroblasts
tissue
hTERT immortalised human skin fibroblasts
phenotype
Non-tumourigenic
treatment
No treatment
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as detailed by Burney et al Stem Cells 2013 31(9):1868-80 Paired end libraries were constructed using standard Illumina protocols, with some modifications. Agencourt AMPure XP (Beckman Coulter) at 0.8x ratio were used to size select out adapter dimers. The Illumina Phusion enzyme was replaced by Kapa HiFi HotStart ready mix (Kapa Biosystems). An Invitrogen SizeSelect R-gel system (Life Technologies) was used to size select following PCR amplification. Sequenced was performed on an Illumina HiSeq 2500 at The Crick Institute, London.