Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

Hematopoietic stem cells (HSC)

cell type

Murine bone marrow derived primary HSCs

cell surface marker

SP-KSL-CD150+

strain

C57BL/6

chip antibody

Abcam ab9050

age

4 month

ChIP

H3K36me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Genomic DNA were extracted from the cells and fragmented to 200-500 bp via biorupter. Followed by immunoprecipittaion with specific antibody, ChIPed DNA were successfully made library using ThruPLEX preparation kit without extra amplification (Rubicon, Ann Arbor, MI)

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

20296427

Reads aligned (%)

84.4

Duplicates removed (%)

66.2

Number of peaks

137 (qval < 1E-05)

mm9

Number of total reads

20296427

Reads aligned (%)

84.3

Duplicates removed (%)

66.4

Number of peaks

132 (qval < 1E-05)