GSM2695609: H3K27ac RAW KO 1h; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
RAW 264.7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
Macrophages
cell type
RAW 264.7 Macrophages
genotype
Parp14 -/-
strain
BALB/c
chip antibody
H3K27ac (abcam, ab4729)
treatment
treated 1h LPS
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with specific antibody. DNA libraries were prepared for HiSeq2000 sequencing as previously described (Ostuni, 2013).