GSM1155147: T47D-overexpressing ELF5-input DNA-24hour; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T47D cell line
cell line
T47D
treatment
doxycycline
timepoint
24h
enrichment
input DNA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed 2 times with cold PBS and scraped into 600 μl PBS with protease inhibitors (P8340, Sigma). Fixed cells were spun for 2 minutes at 6,000xg, washed as before and snap frozen in liquid nitrogen. ChIP-Seq as previously described (doi:10.1016/j.ymeth.2009.03.001) using a 50%–50% mixture of V5-specific antibody and the Santa Cruz N20 anti ELF5 antibody, or no antibody for the input files. DNA was processed for Illumina sequencing using 36-bp reads on a GAIIx.