Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Larvae
Cell type
L4
NA
NA

Attributes by original data submitter

Sample

source_name
N2_whole_worm
genotype
N2
developmental stage
L4
chip antibody
H3K4me3 (Millipore, 17-614, lot:2756917)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked protein-DNA complex were isolated with antibody. ChIP-seqw libraries were prepared for sequencing using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce11

Number of total reads
24188927
Reads aligned (%)
95.3
Duplicates removed (%)
18.8
Number of peaks
5481 (qval < 1E-05)

ce10

Number of total reads
24188927
Reads aligned (%)
95.3
Duplicates removed (%)
18.8
Number of peaks
5475 (qval < 1E-05)

Base call quality data from DBCLS SRA