Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Embryo
Cell type
Embryonic pancreas
NA
NA

Attributes by original data submitter

Sample

source_name
Ngn3-eGFP+ e16.5 cells
developmental stage
e16.5
tissue
pancreas
cell type
Ngn3-eGFP+ cells
strain
Ngn3-eGFP

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells resuspended in transposase-containing reaction mixture with 0.05% digitonin before tagmentation at 37 degrees C for 30 minutes with 300rpm agitation. DNA was then purified with a Qiagen MinElute reaction cleanup kit. As described in Buenrostro JD et al., 2013 Nature Methods

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
31316707
Reads aligned (%)
93.0
Duplicates removed (%)
26.5
Number of peaks
12913 (qval < 1E-05)

mm9

Number of total reads
31316707
Reads aligned (%)
92.8
Duplicates removed (%)
26.6
Number of peaks
12833 (qval < 1E-05)

Base call quality data from DBCLS SRA