GSM2688952: TFIIB ChIP-seq C37 control rep2; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
late embryonic stage
Attributes by original data submitter
Drosophila TFIIB antibody from Jim Kadonaga (UCSD).
Sequenced DNA Library
After fixation, the cells were washed and sonicated using a Bioruptor (Diagenode) to generate chromatin extract. Chromatin extract was mixed with Drosophila virilis chromatin, cleared by centrifugation and used in ChIP with anti-Drosophila TFIIB antibody, pre-bound to a mix of Protein A and G Dynabeads. TFIIB bound DNA was purified, and libraries prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina. Illumina sequencing was performed at SciLifeLab, Stockholm, Sweden.