Antigen

Antigen Class

TFs and others

Antigen

TfIIB

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

S2 cells

chip antibody

Drosophila TFIIB antibody from Jim Kadonaga (UCSD).

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After fixation, the cells were washed and sonicated using a Bioruptor (Diagenode) to generate chromatin extract. Chromatin extract was mixed with Drosophila virilis chromatin, cleared by centrifugation and used in ChIP with anti-Drosophila TFIIB antibody, pre-bound to a mix of Protein A and G Dynabeads. TFIIB bound DNA was purified, and libraries prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina. Illumina sequencing was performed at SciLifeLab, Stockholm, Sweden.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

93786456

Reads aligned (%)

81.9

Duplicates removed (%)

8.9

Number of peaks

0 (qval < 1E-05)

dm3

Number of total reads

93786456

Reads aligned (%)

82.2

Duplicates removed (%)

8.8

Number of peaks

0 (qval < 1E-05)