GSM2684856: H3K9me3 ChIPseq in AtT-20 expressing Neo; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Neural
Cell type
AtT-20
Tissue
Pituitary
Disease
Tumor
Attributes by original data submitter
Sample
source_name
AtT-20 cells expressing 3x flag-Neo
cell line
AtT-20 cells
cell type
pituitary corticotroph cell
passages
10-20
genotype/variation
expressing 3x flag-Neo
chip antibody
H3K9me3 millipore 07-442
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei lysates were sonicated and protein-DNA complexes were isolated with specific antibodies. Fragments of 200-400bp were kept for library preparation Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2000 following the manufacturer's protocols to obtain 50bp paired end reads.