GSM2683319: H3K27Ac for Tnm2 with DMSO Rep 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Lung
Cell type
Lung adenocarcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
Lung adenocarcinoma cells
drugs treated
DMSO
chip-antibody
H3K27 acetylation (Abcam, ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei with Covaris M220 and histone-DNA complexes were isolated with antibody. Libraries were prepared using NEBNext Ultra DNA Library preparation kits from 2-10 ng immunoprecipitated DNA according to manufacturer’s protocol. The finished double strand DNA (dsDNA) libraries were quantified by PicoGreen and Agilent Bioanalyzer according to manufacturer’s protocols. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on an Illumina NextSeq500 with single or paired-end 75bp reads.