Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Primary human monocyte-derived macrophages

tissue

peripheral blood

cell type

monocytes

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Nuclei were pelleted by centrifugation for 5 min at 500g, 4°C. Assay for transposase-accessible chromatin using sequencing (ATAC-seq) protocol developed by (Buenrostro et al., 2013); single end protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

258983209

Reads aligned (%)

74.2

Duplicates removed (%)

62.7

Number of peaks

80393 (qval < 1E-05)

hg19

Number of total reads

258983209

Reads aligned (%)

74.0

Duplicates removed (%)

63.2

Number of peaks

79911 (qval < 1E-05)