GSM2677379: H460 p53 ChIPseq, 2hrs 4GY; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP53
Cell type
Cell type Class
Lung
Cell type
NCI-H460
Primary Tissue
Lung
Site of Extraction
Pleura
Tissue Diagnosis
Carcinoma Large Cell
Attributes by original data submitter
Sample
source_name
H460
cell line
H460
treated with
4GY for 2hrs
chip antibody
anti-p53 antibody (DO-1, Santa Cruz Biotechnology, SC-126X, Lot:G0115)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq lysates were clarified from sonicated nuclei and IPed with DO-1 anti-p53 antibody. RNA samples were extracted with Trizol, purified on Zymo columns, and 500ng polyA selected. DNA and mRNA libraries were prepared with standard illumina compatible protocols (NEBnext Kit), ATACseq Lib was prepared by PCR of MuA tagmented DNA.