Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

J-Lat A72

NA

NA

Sample

source_name

J-Lat A72

treatment

JQ1

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Genomic DNA was extracted using the QIAquick PCR Purification Kit (Qiagen). RNA was isolated via RNeasy Plus Mini Kit (Qiagen). ChIP libraries were generate using the Ovation Ultralow Library Systems V2 (NuGEN). ATAC libraries were generated using the Nextera DNA Sample Prep Kit (Illumina). RNA libraries were generated using the Ovation RNA-Seq System V2 (NuGEN).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

100789168

Reads aligned (%)

92.7

Duplicates removed (%)

41.6

Number of peaks

9803 (qval < 1E-05)

hg19

Number of total reads

100789168

Reads aligned (%)

92.1

Duplicates removed (%)

42.2

Number of peaks

8690 (qval < 1E-05)