Antigen

Antigen Class

TFs and others

Antigen

8-Hydroxydeoxyguanosine

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

normal breast cells

cell line

MCF10A

cell type

normal breast cells

chip antibody

8-Hydroxydeoxyguanosine (AB5830 Millipore)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA from MCF10A cells or from growing MEFs was extracted by using Dneasy Blood&Tissue kit. Genomic DNA were sonicated and immunoprecipitated with antibodies anti 8-oxodG O/N. Eluted DNA was processed for library preparation. For ChIp_Seq anti gH2AX, lysates were clarified from sonicasted nuclei and histone-DNA complexes were isolated with specific antibody. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

52321465

Reads aligned (%)

83.5

Duplicates removed (%)

43.5

Number of peaks

90551 (qval < 1E-05)

hg19

Number of total reads

52321465

Reads aligned (%)

85.1

Duplicates removed (%)

11.3

Number of peaks

2706 (qval < 1E-05)