Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Cardiovascular

Cell type

Heart Ventricles

MeSH Description

The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.

Sample

source_name

heart ventricles from 8 week old mouse

strain

C57/Bl6N

age

8 week

tissue

Heart ventricles

antibody

PolII

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was isolated, immunoprecipitated using antibodies against MEF2 or RNA Polymerase II, and DNA was isolated. Kapa Hyper Prep Kit (KK8500, Roche) according to the manufacturer’s instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

20163033

Reads aligned (%)

82.7

Duplicates removed (%)

49.0

Number of peaks

292 (qval < 1E-05)

mm9

Number of total reads

20163033

Reads aligned (%)

82.5

Duplicates removed (%)

49.1

Number of peaks

292 (qval < 1E-05)