Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa cells
treatment
non-targeting control siRNA
antibody
Pol II Active Motif 39097
cell line
HeLa

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was isolated from crosslinked sheared chromatin subject to immunoprecipitation with the Pol II antibody ChIP–seq samples were sequenced using the Illumina Solexa Hiseq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
28587991
Reads aligned (%)
95.4
Duplicates removed (%)
15.5
Number of peaks
21300 (qval < 1E-05)

hg19

Number of total reads
28587991
Reads aligned (%)
95.0
Duplicates removed (%)
15.9
Number of peaks
21259 (qval < 1E-05)

Base call quality data from DBCLS SRA