Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

ChIP-seq

chip antibody

sc-899X

cell line

293 Flp-In

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Illumina's TruSeq ChIP library Preparation Kit according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

34629959

Reads aligned (%)

70.5

Duplicates removed (%)

30.3

Number of peaks

14192 (qval < 1E-05)

hg19

Number of total reads

34629959

Reads aligned (%)

70.1

Duplicates removed (%)

30.5

Number of peaks

14042 (qval < 1E-05)