Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Breast cancer cells
tissue type
ER+/PR+ MCF7 cell models
er/pr status
ER+/PR+
drug treatment
10nM Estradiol + 10 nM Dexamethasone
hormone exposure time
75 minutes (15 min E2 and 60 min E2+Dexamethasone)
chip antibody
anti-ER (SC biotech HC-20, cat# SC-543, lot# F0515)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method (ChIP and ChIP-sequencing). ChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq) of the paper.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
223517069
Reads aligned (%)
9.9
Duplicates removed (%)
85.2
Number of peaks
16263 (qval < 1E-05)

hg38

Number of total reads
223517069
Reads aligned (%)
11.1
Duplicates removed (%)
83.5
Number of peaks
15741 (qval < 1E-05)

Base call quality data from DBCLS SRA