Antigen

Antigen Class

TFs and others

Antigen

TET2

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

human colorectal cancer cell line

fraction

TET2_bound

antibody

anti TET2 (SantaCruz, catalog# sc-136926, lot# H2412)

cell line

HCT116 colorectal cancer cell line

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For hmeDIP-seq, Illumina libraries were prepared from sonicated genomic DNA followed by immunoprecipitation with anti-5hmC antibody (Active Motif, 39791) and sequenced. For TET2 ChIP-seq, formaldehyde cross-linked material was immunoprecipitated with anti-TET2 antibody (SantaCruz sc-136926) followed by Illumina adapter ligation and sequencing.

Sequencing Platform

instrument_model

Illumina HiScanSQ

hg38

Number of total reads

95678446

Reads aligned (%)

72.8

Duplicates removed (%)

69.2

Number of peaks

1638 (qval < 1E-05)

hg19

Number of total reads

95678446

Reads aligned (%)

72.2

Duplicates removed (%)

71.2

Number of peaks

1580 (qval < 1E-05)