Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Neural
Cell type
Retinal progenitors cells
NA
NA

Attributes by original data submitter

Sample

source_name
retinal cells
cell type
retinal progenitor cells (RPCs)
developmental time point
embryonic day 14
strain
CD1

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Open chromatin extraction, tagmentation and profiling (ATAC-Seq) performed based on Buenrostro et al. (2013); RNA-Seq extraction performed with Qiagen RNAeasy Mini kit; chromatin extraction, crosslinking, shearing and TF / histone- DNA-complexes were isolated with antibodies (ChIP-Seq) based on (Shang et al. 2000) ChIP-Seq; RNA-Seq; ATAC-Seq; Libraries prepared according to the Manufacturer's protocols: TruSeq Nano DNA Library Prep Kit, TruSeq total RNA Sample preparation kit and Nextera DNA sample preparation kit, Illumina® respectively.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
197190049
Reads aligned (%)
92.5
Duplicates removed (%)
13.8
Number of peaks
83835 (qval < 1E-05)

mm9

Number of total reads
197190049
Reads aligned (%)
92.4
Duplicates removed (%)
13.8
Number of peaks
83846 (qval < 1E-05)

Base call quality data from DBCLS SRA