Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Embryonic fibroblast
Cell type
3T3-L1
Tissue
Embryo
Cell Type
Fibroblast

Attributes by original data submitter

Sample

source_name
3T3_L1
cell line
3T3-L1
genotype/variation
knock-down of GPS2
chip antibody
Pol2 (ac-055-100-adgenode mouse)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The experiments were performed as previously described (Cardamone et al, Cell Reports 2014) The library construction protocol followed the manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
23931842
Reads aligned (%)
85.8
Duplicates removed (%)
40.2
Number of peaks
3033 (qval < 1E-05)

mm9

Number of total reads
23931842
Reads aligned (%)
85.6
Duplicates removed (%)
40.3
Number of peaks
3017 (qval < 1E-05)

Base call quality data from DBCLS SRA