Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Embryonic fibroblast

Cell type

3T3-L1

Tissue

Embryo

Cell Type

Fibroblast

Sample

source_name

3T3_L1

cell line

3T3-L1

genotype/variation

control

chip antibody

Pol2 (ac-055-100-adgenode mouse)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The experiments were performed as previously described (Cardamone et al, Cell Reports 2014) The library construction protocol followed the manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

21318056

Reads aligned (%)

81.8

Duplicates removed (%)

48.7

Number of peaks

2730 (qval < 1E-05)

mm9

Number of total reads

21318056

Reads aligned (%)

81.7

Duplicates removed (%)

48.8

Number of peaks

2761 (qval < 1E-05)