SRX2880084
GSM2648375: cKit- H3.3; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3.3

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
Mouse Embryonic Fibroblasts
cell fate transition type
Transdifferentiation
time-point
Fully Transdifferentiated
antibody
H3.3 (Millipore, 17-10245, Lot 2626485)
sorting
cKit-

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
55645105
Reads aligned (%)
95.2
Duplicates removed (%)
13.1
Number of peaks
261 (qval < 1E-05)

mm9

Number of total reads
55645105
Reads aligned (%)
95.1
Duplicates removed (%)
13.6
Number of peaks
249 (qval < 1E-05)

Base call quality data from DBCLS SRA