Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Brain

MeSH Description

The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.

Sample

source_name

Bulk forebrain

Stage

P56

chip antibody

None

genotype

WT

strain

C57BL/6N

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed on cross-linked adult mouse forebrain following established protocols using Chd8 antibody (ab114126; Abcam). Two biological replicates were generated with matched input control libraries generated and sequenced alongside IP libraries. Libraries were prepped using the NuGen Ovation Ultralow Library System V2, and pooled and single end 50 sequenced on one lane of a HiSeq 4000.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

249539984

Reads aligned (%)

98.9

Duplicates removed (%)

30.4

Number of peaks

857 (qval < 1E-05)

mm9

Number of total reads

249539984

Reads aligned (%)

98.7

Duplicates removed (%)

30.4

Number of peaks

997 (qval < 1E-05)