GSM2641019: Lu130 Suz12 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SUZ12
Cell type
Cell type Class
Lung
Cell type
Lu-130
NA
NA
Attributes by original data submitter
Sample
source_name
Lu130_Suz12_ChIPSeq
cell line
Lu130
cell type
Small cell lung cancer cell line
chip antibody
Suz12 (D39F6, Cell Signaling, rabbit monoclonal)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and were prepared for ChIP. Briefly, cells were crosslinked with 1% formalin for 10 min, and crosslinked cell lysates underwent fragmentation by sonication and incubated with antibodies bound to protein A-sepharose beads (50% slurry) overnight at 4 ºC. The beads were washed several times and eluted with elution buffer (1% SDS, 0.1 M NaHCO3). The eluates were treated with 1.5 μg of pronase 2 hr at 42 ºC then incubated at 65 ºC over night to reverse the crosslinks. The ChIP’ed DNA was purified by phenol/chroloform treatment and precipitated with LiCl and 70% ethanol. Sample preparation for ChIP-seq was performed according to the manufacturer's instructions (Illumina). Briefly, size fractionated DNA was extracted and a single adenosine was added using Klenow exo– (3' and 5' exo minus; Illumina). Illumina adaptors were then added and DNA was subjected to 20 cycles of PCR according to manufacturer's instructions.