Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pancreas

Cell type

Pancreatic ductal adenocarcinoma

NA

NA

Sample

source_name

T3/FOXA1-GATA5 organoid

strain

C57BL/6

cell type

Mouse PDA primary tumor organoid (T3)

peturbation

Transduced with MSCV/V5-FOXA1-(P2A)-FLAG-GATA5 retrovirus

antibody

H3K27ac (ab4729; Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were prepared from ~1 millon single cell dissociated of organoid cultures, or ~5 million of monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared according to Illumina's instructions of TruSeq ChIP Sample Prep kit.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

36416719

Reads aligned (%)

98.6

Duplicates removed (%)

37.2

Number of peaks

37315 (qval < 1E-05)

mm9

Number of total reads

36416719

Reads aligned (%)

98.5

Duplicates removed (%)

37.2

Number of peaks

37317 (qval < 1E-05)