Monolayer-cultured cells established from mouse PDA primary tumor organoid (T3)
peturbation
Transduced with MSCV/emp retrovirus
biological replicate
rep1
antibody
H3K27ac (ab4729; Abcam)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from ~1 millon single cell dissociated of organoid cultures, or ~5 million of monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared according to Illumina's instructions of TruSeq ChIP Sample Prep kit.