Antigen

Antigen Class

TFs and others

Antigen

Brd4

Cell type

Cell type Class

Adipocyte

Cell type

Brown preadipocytes

NA

NA

Sample

source_name

Brown preadipocyte cell

cell type

immortalized Mll3-/-; Mll4f/f brown preadipocytes

stage of adipogenesis or myogenesis

Confluent preadipocytes (D0)

strain

C57BL/6J

chip antibody

Brd4 (Bethyl, A301-985A100, lot 3)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

51449503

Reads aligned (%)

89.6

Duplicates removed (%)

22.1

Number of peaks

12593 (qval < 1E-05)

mm9

Number of total reads

51449503

Reads aligned (%)

89.3

Duplicates removed (%)

22.1

Number of peaks

12448 (qval < 1E-05)