Antigen

Antigen Class

TFs and others

Antigen

Brd4

Cell type

Cell type Class

Adipocyte

Cell type

Brown preadipocytes

NA

NA

Sample

source_name

Brown preadipocyte cell

cell type

immortalized Pparg-flox/flox brown preadipocytes

stage of adipogenesis or myogenesis

Immature adipocytes (D2)

strain

C57BL/6J

chip antibody

Brd4 (Bethyl, A301-985A100, lot 3)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

18027377

Reads aligned (%)

82.0

Duplicates removed (%)

18.0

Number of peaks

10704 (qval < 1E-05)

mm9

Number of total reads

18027377

Reads aligned (%)

81.8

Duplicates removed (%)

18.1

Number of peaks

11158 (qval < 1E-05)