Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mouse embryonic stem cells

cell type

ES

passages

15-20

chip antibody

H3K27ac (Abcam, ab4729)

genotype

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-Seq: 10min formaldehyde fixation followed by sonication (histone marks) or Mnase digestion (transcription factors). Immunoprecipitation was performed using ChIP-IT kit (#53040, Active Motif) following manufacturer's protocol. Experiments were done in duplicates. Libraries prepared using Microplex library Preparation kit v2 (C05010014, Diagenode) following manufacturer's protocol (V2 02.15). Libraries were size-selected (200-400bp) and clenaed-up using Agencourt AMPure XP beads (#A63881, Beckman).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

36276285

Reads aligned (%)

99.1

Duplicates removed (%)

26.2

Number of peaks

22059 (qval < 1E-05)

mm9

Number of total reads

36276285

Reads aligned (%)

98.9

Duplicates removed (%)

26.3

Number of peaks

22053 (qval < 1E-05)