GSM2627621: Idx47 Stomach SNU1; Homo sapiens; Bisulfite-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq
Cell type
Cell type Class
Digestive tract
Cell type
SNU-1
Primary Tissue
Stomach
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Stomach cancer cell line
histopathology
adenocarcinoma
cell line
SNU1
Sequenced DNA Library
library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
Each genomic DNAs are obtained from other groups and extracted using Quiagen genomic DNA extraction Kit (69504) Entire amplicons obtained from eight rounds of multiplex PCR were pooled together in equal volumes. For sequencing library construction, we performed a series of enzymatic reactions such as 5’-end phosphorylation, adaptor ligation, and two additional cycles of PCR to attach barcode and other modules, as described elsewhere (Min et al., 2016). Every intermittent purification was conducted using ExpinTM PCR (GeneAll). The sequencing libraries were pooled with equal amount and applied to a multiple parallel sequencing in the same flow cell using Illumina Hi-seq 2500.