Adult differentiated jejunal epithelial cells; middle 1/3 of intestine
genotype
Cdx2f/f; Villin-cre
protocol
Villi from middle 1/3rd (jejunum) of mouse small intestine were isolated by incubating in 5mM EDTA in PBS for 45 minutes at 4°C. Cells were immediately snap-frozen in liquid nitrogen for subsequent ChIP analysis.
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Villus chromatin digested with MNase was incubated overnight with 5 ul H3K4me2 antibody (EMD Millipore, 07-030) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.