Antigen

Antigen Class

Histone

Antigen

H4K16ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

E14 ES cells, CHX, H4K16ac ChIP

cell line

E14

cell type

embryonic stem (ES) cells

treatment

cycloheximide

chip_antibody

H4K16ac (Millipore 07-329, Lot #2639926)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

H4K16ac ChIPs were performed according to the recommendations of the Diagenode low-cell ChIP kit (C01010072). ChIP-seq libraries were prepared using NEBNext ChIPseq library prep kit E6200L using NEB index primers 2, 4, 5, 6, 7, and 12.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

19715033

Reads aligned (%)

94.9

Duplicates removed (%)

12.3

Number of peaks

389 (qval < 1E-05)

mm9

Number of total reads

19715033

Reads aligned (%)

94.7

Duplicates removed (%)

12.4

Number of peaks

392 (qval < 1E-05)