Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Neural

Cell type

Glioblastoma stem-like cells

NA

NA

Sample

source_name

glioblastoma surgical resection

cell type

glioblastoma stem-like cells, transduced with shControl

tumor stage

human glioblastoma multiformis

chip antibody

H3K27me3 (Millipore, 07-449)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq libraries were constructed with the DNA Seq sample prep. kit (ref. IP-102-1001) from Illumina according to the manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

222731021

Reads aligned (%)

94.2

Duplicates removed (%)

25.5

Number of peaks

6492 (qval < 1E-05)

hg19

Number of total reads

222731021

Reads aligned (%)

93.3

Duplicates removed (%)

27.0

Number of peaks

5303 (qval < 1E-05)