SRX2755488
GSM2587554: MEF GETMS H3K4me2 ChIP-seq; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
GETMS
cell type
Mouse Embryonic Fibroblasts (MEF)
treatment
3 days of reprogramming by GETSM
chip antibody
H3K4me2 (Millipore, 07-030)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Chromatin immunoprecipitation (ChIP) assay was performed as previously described (Mendenhall et al., 2013) using H3K27ac antibody (Abcam, ab4729) and H3K4me2 antibody (Millipore, 07-030).

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
31167914
Reads aligned (%)
98.6
Duplicates removed (%)
3.8
Number of peaks
43716 (qval < 1E-05)

mm9

Number of total reads
31167914
Reads aligned (%)
98.5
Duplicates removed (%)
3.8
Number of peaks
43676 (qval < 1E-05)

Base call quality data from DBCLS SRA