Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Prdm15

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mESCs cultured in 2i+LIF
cell type
ESCs
passages
30-40
strain
C57BL/6
chip antibody
PRDM15 antibody (in house)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Single read - Illumina

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
40985399
Reads aligned (%)
94.7
Duplicates removed (%)
44.4
Number of peaks
30862 (qval < 1E-05)

mm9

Number of total reads
40985399
Reads aligned (%)
94.3
Duplicates removed (%)
44.3
Number of peaks
30931 (qval < 1E-05)

Base call quality data from DBCLS SRA