Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
J1 cells
antibodies
None
cell type
J1 cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitated DNA was extracted with phenol-chloroform, ethanol-precipitated and treated with RNase. ChIP-Seq was carried out using antibodies against RNA PolymeraseII or H3K4me3, as previously described (Goren et. al. (2010))

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
31669629
Reads aligned (%)
92.2
Duplicates removed (%)
16.6
Number of peaks
765 (qval < 1E-05)

mm9

Number of total reads
31669629
Reads aligned (%)
91.9
Duplicates removed (%)
16.5
Number of peaks
871 (qval < 1E-05)

Base call quality data from DBCLS SRA