Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KAT2A

Cell type

Cell type Class
Neural
Cell type
U-251 MG
Primary Tissue
Brain
Tissue Diagnosis
Astrocytoma

Attributes by original data submitter

Sample

source_name
U251
antibody
Gcn5

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The libraries were amplified by using three cycles of PCR and then assessed for quality by using a Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies, Ankeny, IA) and quantified by using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
70025351
Reads aligned (%)
64.0
Duplicates removed (%)
5.4
Number of peaks
2697 (qval < 1E-05)

hg38

Number of total reads
70025351
Reads aligned (%)
65.4
Duplicates removed (%)
4.2
Number of peaks
2669 (qval < 1E-05)

Base call quality data from DBCLS SRA