Antigen

Antigen Class

Histone

Antigen

H3K79suc

Cell type

Cell type Class

Neural

Cell type

U-251 MG

Primary Tissue

Brain

Tissue Diagnosis

Astrocytoma

Sample

source_name

U251

antibody

H3K79succ

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The libraries were amplified by using three cycles of PCR and then assessed for quality by using a Fragment Analyzer High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies, Ankeny, IA) and quantified by using a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

67873412

Reads aligned (%)

80.2

Duplicates removed (%)

3.4

Number of peaks

1659 (qval < 1E-05)

hg19

Number of total reads

67873412

Reads aligned (%)

79.5

Duplicates removed (%)

5.0

Number of peaks

1758 (qval < 1E-05)