GSM2582375: ChIPseq for HP1g in wt mES cells, rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Cbx3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
embryonic stem cells, HP1g ChIP
cell type
embryonic stem (ES) cells
genetic background
hybrid 129-C57BL/6
genotype/variation
Cbx3 FlagAvi
treatment
none
chip antibody
Bio ChIP, Streptavidin Dynabeads (Thermofisher)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10^7 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 1% of chromatin was set aside as Input control. The chromatin was incubated with Streptavidin-coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ostapcuk et al, 2017. Libraries were prepared using the NEB Next DNA library prep kit according to manufacturer's instructions. For barcodes, see sample characteristics.