Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Others

Cell type

Embryonic fibroblasts

NA

NA

Sample

source_name

embryonic fibroblast

reprogramming stage

hiF-T

chip antibody

H3K9me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA DNA Library Preparation Kits.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

15901414

Reads aligned (%)

79.6

Duplicates removed (%)

3.8

Number of peaks

4153 (qval < 1E-05)

hg19

Number of total reads

15901414

Reads aligned (%)

77.6

Duplicates removed (%)

4.6

Number of peaks

3688 (qval < 1E-05)