Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Others

Cell type

Embryonic fibroblasts

NA

NA

Sample

source_name

embryonic fibroblast

reprogramming stage

hiF-T

chip antibody

H3K27me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA DNA Library Preparation Kits.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

9023834

Reads aligned (%)

86.0

Duplicates removed (%)

1.7

Number of peaks

330 (qval < 1E-05)

hg19

Number of total reads

9023834

Reads aligned (%)

85.3

Duplicates removed (%)

1.8

Number of peaks

187 (qval < 1E-05)