Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Erythroid Cells

MeSH Description

The series of cells in the red blood cell lineage at various stages of differentiation.

Sample

source_name

Ter119+ Erythroid cells

strain

C57BL6

tissue

Phenylhydrazine treated spleen

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

A single cell suspension was made by gently dissociating the spleen of a phenyhydrazine treated mouse and passing it through a 70um filter. Ter119 selection was performed by staining the cells with phycoerythrin conjugated anti-ter119 antibodies (BD biosciences). The cells were subsequently washed and then incubated with MACS anti-phycoerythrin beads. Cells were then separated using an auto-MACS (Miltenyi Biotec). Illumina Nextera DNA library kit, subjected to ATAC-seq (Assay for Transposase-Accessible Chromatin)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

41549221

Reads aligned (%)

97.5

Duplicates removed (%)

15.3

Number of peaks

13087 (qval < 1E-05)

mm9

Number of total reads

41549221

Reads aligned (%)

97.4

Duplicates removed (%)

15.5

Number of peaks

13073 (qval < 1E-05)