Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mESC
genotype
UTX Knockout
chip antibody
Anti-H3K27me3 (Upstate, 07-449)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
38525142
Reads aligned (%)
92.4
Duplicates removed (%)
14.3
Number of peaks
3842 (qval < 1E-05)

mm9

Number of total reads
38525142
Reads aligned (%)
92.2
Duplicates removed (%)
14.4
Number of peaks
3902 (qval < 1E-05)

Base call quality data from DBCLS SRA