Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESC

genotype

UTX Wildtype

chip antibody

Anti-H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer’s recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

39974983

Reads aligned (%)

90.7

Duplicates removed (%)

13.6

Number of peaks

10199 (qval < 1E-05)

mm9

Number of total reads

39974983

Reads aligned (%)

90.5

Duplicates removed (%)

13.7

Number of peaks

10397 (qval < 1E-05)