Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Lung
Cell type
MRC-5
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
MRC5 Day 5 after OSvK rep1
chip antibody
H3K27me3 (Abcam ab6002 Lot: GR218433-1)
cell line
MRC5 fibroblast
protocol
Oct4, Sox2-VP16 and Klf4 addition
time
5 days

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
See ChIP-Endo Protocol.pdf

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
5566077
Reads aligned (%)
92.9
Duplicates removed (%)
2.1
Number of peaks
90 (qval < 1E-05)

hg19

Number of total reads
5566077
Reads aligned (%)
91.5
Duplicates removed (%)
2.0
Number of peaks
90 (qval < 1E-05)

Base call quality data from DBCLS SRA